MLN51/CASC3 Polyclonal antibody

MLN51/CASC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18047-1-AP

Synonyms

CASC3, MLN51, MLN 51, CASC 3, Btz

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Validation Data Gallery

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  WB analysis using 18047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18047-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 18047-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18047-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18047-1-AP (MLN51/CASC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18047-1-AP

  Immunofluorescent analysis of HepG2 cells, using CASC3 antibody 18047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A2780 cells, MCF-7 cells, SKOV-3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

18047-1-AP targets MLN51/CASC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLN51/CASC3 fusion protein Ag12694
• Full Name: cancer susceptibility candidate 3
• Calculated Molecular Weight: 703 aa, 76 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC050526
• Gene Symbol: CASC3
• Gene ID (NCBI): 22794
• RRID: AB_10640534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay.; Authors - Ivone G Bruno
human	WB,IF	Mol Biol Cell; Perispeckles are major assembly sites for the exon junction core complex.; Authors - Daguenet Elisabeth E