GORASP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GORASP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

65

Rezensionen (4)

Kat-Nr. : 10598-1-AP

Synonyme

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

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Galerie der Validierungsdaten

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  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10598-1-AP

  WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells.

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  WB analysis of rat testis using 10598-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10598-1-AP

  IP result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.

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  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10598-1-AP (GORASP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10598-1-AP (GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Raji-Zellen, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 25 publications below
• WB: See 47 publications below
• IHC: See 2 publications below
• IF: See 31 publications below

Produktinformation

10598-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GORASP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GORASP2 fusion protein Ag0950
• Vollständiger Name: golgi reassembly stacking protein 2, 55kDa
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC007770
• Gene symbol: GORASP2
• Gene ID (NCBI): 26003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Publikationen

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	WB,IF	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	WB	Dev Cell; A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.; Authors - Francesco Ferraro ; KD Validated
		Nat Commun; Land-locked mammalian Golgi reveals cargo transport between stable cisternae.; Authors - Myun Hwa Dunlop
mouse	IF	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk ; KD Validated
human	WB,IF	J Cell Biol; GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.; Authors - Xiang Yi Y ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cassan (Verified Customer) (06-16-2022)

Verified good working antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: brain

GORASP2 Antibody Immunofluorescence validation (1 to 200 dilution) in brain (Cat no:10598-1-AP)

FH

Hai (Verified Customer) (07-09-2021)

I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Melanoma cells

GORASP2 Antibody Immunofluorescence validation (1:400 dilution) in Melanoma cells (Cat no:10598-1-AP)

FH

Ana (Verified Customer) (03-14-2021)

GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Primary Fibroblasts

GORASP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Primary Fibroblasts (Cat no:10598-1-AP)

FH

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary human fibroblasts

GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)

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