GS28 Polyclonal antibody

GS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16106-1-AP

Synonyms

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 16106-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16106-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16106-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 16106-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16106-1-AP (GS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells, human liver tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16106-1-AP targets GS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GS28 fusion protein Ag9021
• Full Name: golgi SNAP receptor complex member 1
• Calculated Molecular Weight: 28.6 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC012620
• Gene Symbol: GS28
• Gene ID (NCBI): 9527
• RRID: AB_2294912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)