RAB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15420-1-AP

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A

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Validation Data Gallery

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  WB analysis of mouse brain using 15420-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15420-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15420-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15420-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15420-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15420-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, rat brain tissue
• Positive IHC detected in: human brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IF: See 2 publications below

Product Information

15420-1-AP targets RAB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB2 fusion protein Ag7708
• Full Name: RAB2A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC008929
• Gene Symbol: RAB2A
• Gene ID (NCBI): 5862
• RRID: AB_2176874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Cell Host Microbe; A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.; Authors - Cheryl N Miller ; KD Validated
human	WB	Nat Commun; Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells.; Authors - Shingo Kozono
		Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
human	WB	Cell Rep; Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes; Authors - Ariane Schleinitz
		EMBO Rep; RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.; Authors - Hiroaki Kajiho