GRASP65,GORASP1 Polyclonal antibody

GRASP65,GORASP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10747-2-AP

Synonyms

GORASP1, GRASP65, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1

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Validation Data Gallery

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  WB analysis using 10747-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10747-2-AP (GRASP65,GORASP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10747-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GRASP65,GORASP1 antibody (10747-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10747-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GRASP65,GORASP1 antibody (10747-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

10747-2-AP targets GRASP65,GORASP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRASP65,GORASP1 fusion protein Ag1054
• Full Name: golgi reassembly stacking protein 1, 65kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC008928
• Gene Symbol: GRASP65
• Gene ID (NCBI): 64689
• RRID: AB_2113204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)

Publications

Species	Application	Title
human	WB	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human	IF	J Cell Biochem; Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury.; Authors - Guang Ji
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
human	WB	Biochem Biophys Res Commun; Golgi damage caused by dysfunction of PiT-2 in primary familial brain calcification; Authors - Huifang Sun
mouse	WB	Cell Death Dis; GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response; Authors - Theodomir Dusabimana
mouse	WB	Prostaglandins Other Lipid Mediat; GOLPH3 knockdown alleviates the inflammation and apoptosis in lipopolysaccharide-induced acute lung injury by inhibiting Golgi stress mediated autophagy; Authors - Yanru Wang