Geminin Polyklonaler Antikörper

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• KD/KO Validated

Geminin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Kat-Nr. : 10802-1-AP

Synonyme

GMNN, MGORS6, Gem

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Galerie der Validierungsdaten

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  WB analysis using 10802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10802-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10802-1-AP

  WB result of Geminin antibody (10802-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Geminin transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 10802-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10802-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10802-1-AP

  IP result of anti-Geminin (IP:10802-1-AP, 4ug; Detection:10802-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

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  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10802-1-AP (Geminin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of HepG2 using 10802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (10802-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10802-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 10802-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hodengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 12 publications below
• IF: See 37 publications below
• IP: See 1 publications below

Produktinformation

10802-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Geminin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Geminin fusion protein Ag1076
• Vollständiger Name: geminin, DNA replication inhibitor
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 28-29 kDa
• GenBank-Zugangsnummer: BC005185
• Gene symbol: GMNN
• Gene ID (NCBI): 51053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

Publikationen

Species	Application	Title
mouse	IF	Nature; Whole genomes redefine the mutational landscape of pancreatic cancer.; Authors - Nicola Waddell
human	IF	Cancer Discov; Biomarkers Associating with PARP Inhibitor Benefit in Prostate Cancer in the TOPARP-B Trial.; Authors - Suzanne Carreira
human	IF	Ann Oncol; Association of RAD51 with homologous recombination deficiency (HRD) and clinical outcomes in untreated triple-negative breast cancer (TNBC): analysis of the GeparSixto randomized clinical trial.; Authors - A Llop-Guevara
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IF	Nat Commun; Homologous recombination deficiency derived from whole-genome sequencing predicts platinum response in triple-negative breast cancers; Authors - Petra Ter Brugge
human	IF	Sci Transl Med; BRCAness, SLFN11, and RB1 loss predict response to topoisomerase I inhibitors in triple-negative breast cancers.; Authors - Florence Coussy

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