CDT1 Polyclonal antibody

CDT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14382-1-AP

Synonyms

CDT1, DNA replication factor Cdt1, Double parked homolog, DUP, RIS2

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Validation Data Gallery

• 

  WB analysis using 14382-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14382-1-AP (CDT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

14382-1-AP targets CDT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDT1 fusion protein Ag5810
• Full Name: chromatin licensing and DNA replication factor 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC000137
• Gene Symbol: CDT1
• Gene ID (NCBI): 81620
• RRID: AB_2076871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Nature; Skp2 targeting suppresses tumorigenesis by Arf-p53-independent cellular senescence.; Authors - Lin Hui-Kuan HK
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	WB	Nat Commun; IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.; Authors - Hong Lin
human	WB	J Med Chem; Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.; Authors - Wenjuan Zhou
human	WB,IHC	Front Oncol; CDT1 Is a Novel Prognostic and Predictive Biomarkers for Hepatocellular Carcinoma; Authors - Chenhui Cai
rat	IHC	J Clin Biochem Nutr; Involvement of proliferation of atypical hepatocytes and CDT 1 in the liver cancer of rats administered the diethylnitrosamine; Authors - Masahiro Ogawa