MCM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCM2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (1)

Cat no : 10513-1-AP

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

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Validation Data Gallery

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  WB analysis using 10513-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10513-1-AP

  WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 10513-1-AP

  IP result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of human cervical cancer using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human gliomas using 10513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (10513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of NIH/3T3 using 10513-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human cervical cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human gliomas tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 11 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

10513-1-AP targets MCM2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCM2 fusion protein Ag0798
• Full Name: minichromosome maintenance complex component 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 116-125 kDa
• GenBank Accession Number: BC007670
• Gene Symbol: MCM2
• Gene ID (NCBI): 4171
• RRID: AB_2142131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Publications

Species	Application	Title
mouse	WB,IP	Nature; CDC7-independent G1/S transition revealed by targeted protein degradation.; Authors - Jan M Suski
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IF	Nat Commun; Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes; Authors - Hana Polasek-Sedlackova
	WB	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
human	WB,RIP	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mandi (Verified Customer) (03-02-2020)

Biotinylated secondary and Alex Fluor needed for decent signal vs non-specific staining

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat brain