CDC6 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 11640-1-AP

Synonyms

HsCDC18, CDC6-related protein, CDC6 related protein, Cdc18-related protein, CDC18L

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Validation Data Gallery

• 

  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11640-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11640-1-AP

  IP result of anti-CDC6 (IP:11640-1-AP, 4ug; Detection:11640-1-AP 1:1000) with HeLa cells lysate 1360 ug.

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  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CDC6 antibody (11640-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, SMMC-7721 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11640-1-AP targets CDC6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC6 fusion protein Ag2228
• Full Name: cell division cycle 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 560 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC025232
• Gene Symbol: CDC6
• Gene ID (NCBI): 990
• RRID: AB_2078519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Publications

Species	Application	Title
human	WB	Mol Cancer; A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.; Authors - Xiaoyu He
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IF	Proc Natl Acad Sci U S A; Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.; Authors - Yim Hyungshin H
mouse	IF	PLoS Genet; Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.; Authors - Ma Pengpeng P
human	WB	Int J Mol Sci; Norcantharidin Induces Immunogenic Cell Death of Bladder Cancer Cells through Promoting Autophagy in Acidic Culture.; Authors - Lili Xu
human	WB	Aging (Albany NY); β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.; Authors - Kun Zou