Galectin-3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Galectin-3 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 14979-1-AP

Synonyme

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa cells using 14979-1-AP

  WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

• 

  WB analysis using 14979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 14979-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14979-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14979-1-AP

  IP Result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14979-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14979-1-AP (Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14979-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Galectin-3 antibody (14979-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, MCF-7-Zellen, NIH/3T3-Zellen, Ratten-Kolongewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Produktinformation

14979-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Galectin-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Ag6891
• Vollständiger Name: lectin, galactoside-binding, soluble, 3
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC001120
• Gene symbol: LGALS3
• Gene ID (NCBI): 3958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Publikationen

Species	Application	Title
human	IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
mouse	IF	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su
human,mouse	WB	Front Cell Dev Biol; Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.; Authors - Zilong Li
human	WB	J Agric Food Chem; Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.; Authors - Wen Wen Xu
mouse	WB	Int J Biol Sci; Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis.; Authors - Zhenhua Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (07-07-2021)

HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

Galectin-3 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:14979-1-AP)

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