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Galectin-3 Monoclonal antibody
Galectin-3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 60207-1-Ig
Validation Data Gallery
|Positive WB detected in||A431 cells, HSC-T6 cells, HeLa cells, A549 cells, MCF-7 cells, T-47D cells, HepG2 cells, Caco-2 cells, human placenta tissue|
|Positive IHC detected in||human thyroid cancer tissue, human colon tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse kidney tissue, MCF-7 cells, human thyroid cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
60207-1-Ig targets Galectin-3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Galectin-3 fusion protein Ag6753|
|Full Name||lectin, galactoside-binding, soluble, 3|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC001120|
|Gene ID (NCBI)||3958|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kenzo (Verified Customer) (08-29-2023)
I used this antibody in mouse kidney tissues and it worked well.
Muhammad (Verified Customer) (09-13-2022)
Work best in HUVECs
Noémie (Verified Customer) (10-20-2021)
I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.