Validation Data Gallery
|Positive WB detected in||HeLa cells, COLO 320 cells, HEK-293 cells, fetal human brain tissue, HepG2 cells, A549 cells, human milk tissue, human plasma tissue|
|Positive IP detected in||HepG2 cells, HEK-293 cells|
|Positive IHC detected in||human oesophagus cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10281-1-AP targets LGALS3BP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LGALS3BP fusion protein Ag0294|
|Full Name||lectin, galactoside-binding, soluble, 3 binding protein|
|Calculated molecular weight||585 aa, 65 kDa|
|Observed molecular weight||65-90 kDa|
|GenBank accession number||BC002403|
|Gene ID (NCBI)||3959|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).
|Product Specific Protocols|
|WB protocol for LGALS3BP antibody 10281-1-AP||Download protocol|
|IHC protocol for LGALS3BP antibody 10281-1-AP||Download protocol|
|IF protocol for LGALS3BP antibody 10281-1-AP||Download protocol|
|IP protocol for LGALS3BP antibody 10281-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.
Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.
Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles.
MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (03-15-2022)
Beautiful antibody - produces a single band at the correct size.