FAF1 Polyklonaler Antikörper

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• KD/KO Validated

FAF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10271-1-AP

Synonyme

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10271-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  WB result of FAF1 antibody (10271-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF1 transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 10271-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10271-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10271-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen, Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

10271-1-AP bindet in WB, IF, IHC, ELISA FAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAF1 fusion protein Ag0407
• Vollständiger Name: Fas (TNFRSF6) associated factor 1
• Berechnetes Molekulargewicht: 650aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC004970
• Gene symbol: FAF1
• Gene ID (NCBI): 11124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.

Publikationen

Species	Application	Title
human	WB,IF	Cell Death Differ; FAF1 mediates regulated necrosis through PARP1 activation upon oxidative stress leading to dopaminergic neurodegeneration.; Authors - Changsun Yu
human, mouse	WB,IHC,IF	Hum Mol Genet; Accumulation of the parkin substrate, FAF1, plays a key role in the dopaminergic neurodegeneration.; Authors - Sul Jee-Won JW
mouse	WB,IHC,IF	J Neurosci; Fas-associated factor 1 as a regulator of olfactory axon guidance.; Authors - Cheng Kai K
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
human	WB	Cancer Manag Res; MiR-26a-5p Serves as an Oncogenic MicroRNA in Non-Small Cell Lung Cancer by Targeting FAF1.; Authors - Ming-Fan Ye ; KD Validated
mouse	IF	Neuroreport; Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice.; Authors - Jong Mu Kim

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