MIG6; ERRFI1 Polyklonaler Antikörper

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• KD/KO Validated

MIG6; ERRFI1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 11630-1-AP

Synonyme

ERRFI1, Mitogen-inducible gene 6 protein, MIG-6, MIG6, MIG 6

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Galerie der Validierungsdaten

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  WB analysis using 11630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11630-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MIG6; ERRFI1 antibody (11630-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

11630-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MIG6; ERRFI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIG6; ERRFI1 fusion protein Ag2202
• Vollständiger Name: ERBB receptor feedback inhibitor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC025337
• Gene symbol: MIG6
• Gene ID (NCBI): 54206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

Publikationen

Species	Application	Title
human	WB	Nat Biomed Eng; Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors.; Authors - Hong-Won Lee
mouse	IHC	Proc Natl Acad Sci U S A; Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation.; Authors - Ben Staal
human	WB	Nucleic Acids Res; A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells.; Authors - Masahiko Ajiro
human	WB	Aging Cell; METTL14 affects UVB-induced human dermal fibroblasts photoaging via miR-100-3p biogenesis in an m6 A-dependent manner; Authors - Lihao Chen
human	IHC	EMBO Rep; MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer.; Authors - Jiabei He
human	WB	Arch Pharm Res; Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC; Authors - Xiaoping Song ; KD Validated

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