Emerin Polyklonaler Antikörper

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• KD/KO Validated

Emerin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 10351-1-AP

Synonyme

EMD, STA, LEMD5, EDMD

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Galerie der Validierungsdaten

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  WB analysis using 10351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10351-1-AP

  WB result of Emerin antibody (10351-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells.

• 

  WB analysis of HepG2 using 10351-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10351-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10351-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10351-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10351-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10351-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10351-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle tissue using 10351-1-AP

  IP result of EMD antibody (10351-1-AP for IP and Detection) with mouse skeletal muscle tissue lysate.

• 

  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa cells using 10351-1-AP

  IF result of anti-EMD (10351-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

• 

  IF Staining of HeLa using 10351-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (10351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 10351-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Emerin (10351-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 17 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

10351-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Emerin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Emerin fusion protein Ag0236
• Vollständiger Name: emerin
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Publikationen

Species	Application	Title
human	WB,IF	Nature; Cytoplasmic control of intranuclear polarity by human cytomegalovirus.; Authors - Dean J Procter
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
	IF	Cell; DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.; Authors - Matthias Samwer
human	IF,IP	Nat Commun; Off-the-shelf proximity biotinylation for interaction proteomics.; Authors - Irene Santos-Barriopedro
human	IF	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta
human	IF	J Cell Biol; Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.; Authors - Banafsheh Golchoubian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-12-2019)

Antibody recognizes clear band at the corrected expected molecular weight of Emerin.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Emerin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10351-1-AP)

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