• Featured Product
  • KD/KO Validated

BCLAF1 Polyclonal antibody

BCLAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26809-1-AP

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164



Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26809-1-AP targets BCLAF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCLAF1 fusion protein Ag25210
Full Name BCL2-associated transcription factor 1
Calculated Molecular Weight 920 aa, 106 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberBC132780
Gene Symbol BCLAF1
Gene ID (NCBI) 9774
RRIDAB_2880642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

Protocols

Product Specific Protocols
WB protocol for BCLAF1 antibody 26809-1-APDownload protocol
IHC protocol for BCLAF1 antibody 26809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
humanWB

Aging (Albany NY)

LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA.

Authors - Mingwei Chen
humanWB,IHC,IF

Transl Oncol

Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma

Authors - Wenchong Tan
  • KD Validated
mouseWB

eLife

SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing

Authors - Wenbo Liu
humanWB

J Oncol

The Comprehensive Analysis of N6-Methyadenosine Writer METTL3 and METTL14 in Gastric Cancer

Authors - Yiling Ge
humanWB

Biochem Genet

BCLAF1 is Expressed as a Potential Anti-oncogene in Bile Duct Cancer

Authors - Yutong Zhou