BCLAF1 Polyclonal antibody

BCLAF1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26809-1-AP

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164

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Validation Data Gallery

• 

  WB analysis of Jurkat using 26809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26809-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26809-1-AP is BCLAF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCLAF1 fusion protein Ag25210
• Full Name: BCL2-associated transcription factor 1
• Calculated molecular weight: 920 aa, 106 kDa
• Observed molecular weight: 145 kDa
• GenBank accession number: BC132780
• Gene symbol: BCLAF1
• Gene ID (NCBI): 9774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

Publications

Species	Application	Title
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
human	WB	Aging (Albany NY); LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA.; Authors - Mingwei Chen
human	WB,IHC,IF	Transl Oncol; Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma; Authors - Wenchong Tan ; KD Validated
mouse	WB	eLife; SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing; Authors - Wenbo Liu
human	WB	J Oncol; The Comprehensive Analysis of N6-Methyadenosine Writer METTL3 and METTL14 in Gastric Cancer; Authors - Yiling Ge