TMPO/LAP2 Polyclonal antibody

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  WB analysis using 14651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14651-1-AP

  WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells.

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  IP experiment of HeLa using 14651-1-AP

  IP result of anti-TMPO/LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14651-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa cells using 14651-1-AP

  IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

• Featured Product
• KD/KO Validated

TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14651-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LAP2, TMPO, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopoietin-related peptide isoforms beta/gamma, Thymopoietin isoform alpha

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Tested Applications

• Positive WB detected in: HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue, mouse lung tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 5 publications below

Product Information

14651-1-AP targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6247

  Product name: Recombinant human TMPO protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-362 aa of BC053675

  Sequence: MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISAS

• Full Name: thymopoietin
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 75 kDa, 51 kDa and 39 kDa
• GenBank Accession Number: BC053675
• Gene Symbol: TMPO/LAP2
• Gene ID (NCBI): 7112
• RRID: AB_2205440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42167
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	IF	J Cell Biol; A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.; Authors - Tino Pleiner
human	WB	Sci Rep; MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.; Authors - Yuhua Fu
bovine	WB	J Dairy Sci; Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.; Authors - Xiao Ma ; KO Validated
human	WB	World J Surg Oncol; Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.; Authors - Lin Zhang ; KD Validated
mouse	IF	Theranostics; Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.; Authors - Xiaolong Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lauren (Verified Customer) (02-20-2020)

Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

TMPO/LAP2 Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:14651-1-AP)