EBI3 Polyklonaler Antikörper

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• KD/KO Validated

EBI3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12371-1-AP

Synonyme

EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta

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Galerie der Validierungsdaten

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  WB analysis using 12371-1-AP

  human placenta tissue was subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 12371-1-AP

  IP result of anti-EBI3 (IP:12371-1-AP, 4ug; Detection:12371-1-AP 1:800) with human placenta tissue lysate 600 ug.

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  IHC staining of human spleen using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Hausschwein-Milzgewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

12371-1-AP bindet in WB, IP, IF, IHC, ELISA EBI3 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EBI3 fusion protein Ag3051
• Vollständiger Name: Epstein-Barr virus induced 3
• Berechnetes Molekulargewicht: 229 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-35 kDa
• GenBank-Zugangsnummer: BC015364
• Gene symbol: EBI3
• Gene ID (NCBI): 10148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

Publikationen

Species	Application	Title
	WB	J Immunol; Role of IL-6-IL-27 Complex in Host Antiviral Immune Response.; Authors - Qi Zuo
human	WB,IF	PLoS One; Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor; Authors - Tong Zhang
mouse	IF	Pathog Dis; Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.; Authors - Jia-Hui Deng ; KO Validated

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