IL-27A Monoclonal antibody

IL-27A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E1H5

Cat no : 66164-1-Ig

Synonyms

IL-27, IL27, IL 27A, IL 27 subunit alpha, IL 27 A

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66164-1-Ig

  IL27 fusion protein were subjected to SDS PAGE followed by western blot with 66164-1-Ig (IL-27 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66164-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 66164-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Recombinant protein, Ag21436
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66164-1-Ig targets IL-27A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-27A fusion protein Ag21436
• Full Name: interleukin 27
• Calculated Molecular Weight: 243 aa, 27 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC062422
• Gene Symbol: IL-27
• Gene ID (NCBI): 246778
• RRID: AB_2881560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

Publications

Species	Application	Title
human	IHC	Anticancer Res; The Prognostic Significance of Peritumoral Lymphocytes' Band-like Structure in Type II Endometrial Cancer.; Authors - Yusuke Shimizu