Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, RAW 264.7 cells|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, SKOV-3 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10427-2-AP targets Calnexin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, monkey, Hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calnexin fusion protein Ag0697|
|Calculated molecular weight||90 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC003552|
|Gene ID (NCBI)||821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.
|Product Specific Protocols|
|WB protocol for Calnexin antibody 10427-2-AP||Download protocol|
|IHC protocol for Calnexin antibody 10427-2-AP||Download protocol|
|IF protocol for Calnexin antibody 10427-2-AP||Download protocol|
|FC protocol for Calnexin antibody 10427-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guo-rong (Verified Customer) (02-06-2023)
Clear bands can be detected in total cell lysates rather than in extracellular vesicles, as a good EV exclusive marker
Saba (Verified Customer) (03-14-2022)
The band of the antibody was so sharp and specific. The dilution I used was 1:4000 and it worked perfectly.
Meliti (Verified Customer) (09-25-2019)
1:1000 dilution in 5%milk PBST O/N incubation for all antibodies depicted which all come from Proteintech 1:5000 anti rabbit HRP in 5%milk PBST for 1h