Cytochrome c Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytochrome c Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (8)

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

520

Rezensionen (5)

Kat-Nr. : 10993-1-AP

Synonyme

CYCS, CYC

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Galerie der Validierungsdaten

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  WB analysis using 10993-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10993-1-AP

  WB result of Cytochrome c antibody (10993-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.

• 

  WB analysis of HEK-293 using 10993-1-AP

  WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.

• 

  WB analysis using 10993-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10993-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10993-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 10993-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10993-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse eye using 10993-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 10993-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10993-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausnierengewebe, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Rattenlebergewebe, Rattennierengewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 487 publications below
• IHC: See 28 publications below
• IF: See 25 publications below
• FC: See 1 publications below

Produktinformation

10993-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA Cytochrome c und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Huhn, Hund, Maus, Rind, Ziege, Hippospongia
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytochrome c fusion protein Ag1455
• Vollständiger Name: cytochrome c, somatic
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12-15 kDa
• GenBank-Zugangsnummer: BC009578
• Gene symbol: Cytochrome c
• Gene ID (NCBI): 54205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Publikationen

Species	Application	Title
human	WB	Cell; Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.; Authors - Tara E Tracy
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
human	IF	ACS Appl Mater Interfaces; Mitochondrial Voltage-Dependent Anion Channel 1-Hexokinase-II Complex-Targeted Strategy for Melanoma Inhibition Using Designed Multiblock Peptide Amphiphiles.; Authors - Fan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kazu (Verified Customer) (12-14-2022)

Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse optic nerve

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC34

FH

Ying (Verified Customer) (04-22-2021)

good antibody. work well with mouse cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000

Cytochrome c Antibody Western Blot validation (1:4000 dilution) in (Cat no:10993-1-AP)

FH

Chi (Verified Customer) (09-26-2019)

The antibody works very well with good signal and low background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse embryonic fibroblasts

Cytochrome c Antibody Western Blot, validation (1:2000 dilution) in mouse embryonic fibroblasts (Cat no:10993-1-AP)

FH

Xiaoping (Verified Customer) (10-22-2018)

The signal is good and the size is between 10-15kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse fibroblast

Cytochrome c Antibody Western Blot, validation (1:1000 dilution) in mouse fibroblast (Cat no:10993-1-AP)

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