cleaved-Caspase 3 Polyclonal antibody

cleaved-Caspase 3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

130

Cat no : 25546-1-AP

Synonyms

cleaved-Caspase 3

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Validation Data Gallery

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  WB analysis of HepG2 using 25546-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25546-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (cleaved-caspase 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25546-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25546-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25546-1-AP

  IP result of anti-cleaved-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25546-1-AP

  Immunofluorescent analysis of HepG2 cells using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Tunicamycin treated HepG2 cells, Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 110 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Product Information

25546-1-AP targets cleaved-Caspase 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.

Publications

Species	Application	Title
human		Nat Methods; DART-seq: an antibody-free method for global m6A detection.; Authors - Kate D Meyer
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	Diabetes; MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response.; Authors - Lele Ji
		PLoS Biol; The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.; Authors - Hongmei Liu
human	WB	Biol Res; MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma.; Authors - Tingting Wu
rat	WB	Oxid Med Cell Longev; Classic Prescription, Kai-Xin-San, Ameliorates Alzheimer's Disease as an Effective Multitarget Treatment: From Neurotransmitter to Protein Signaling Pathway.; Authors - Sirui Guo