CENPJ Polyklonaler Antikörper

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• KD/KO Validated

CENPJ Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

49

Kat-Nr. : 11517-1-AP

Synonyme

CPAP, LAG-3-associated protein, Centrosomal P4.1-associated protein, Centromere protein J, CENP-J

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11517-1-AP

  WB result of CENPJ antibody (11517-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPJ transfected Jurkat cells.

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  WB analysis of mouse testis using 11517-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 11517-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CENPJ antibody (11517-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 11517-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 41 publications below

Produktinformation

11517-1-AP bindet in WB, IHC, IF/ICC, ELISA CENPJ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPJ fusion protein Ag2078
• Vollständiger Name: centromere protein J
• Berechnetes Molekulargewicht: 1338 aa, 153 kDa
• Beobachtetes Molekulargewicht: 153 kDa
• GenBank-Zugangsnummer: BC024209
• Gene symbol: CENPJ
• Gene ID (NCBI): 55835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Publikationen

Species	Application	Title
human	IF	Nature; TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.; Authors - Franz Meitinger
human	IF	Science; Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.; Authors - Yao Liang Wong
mouse	WB,IF	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao
mouse	WB,IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
rat	IF	Dev Cell; SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication.; Authors - Chii Shyang Fong
human	IF	Nat Commun; Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.; Authors - Søs Grønbæk Holdgaard

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