BID Polyklonaler Antikörper

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• KD/KO Validated

BID Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

58

Kat-Nr. : 10988-1-AP

Synonyme

BID, FP497, p11 BID, p22 BID

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Galerie der Validierungsdaten

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  WB analysis using 10988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10988-1-AP

  WB result of BID antibody (10988-1-AP, 1:8000) with si-Control and si-BID transfected HeLa cells.

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  WB analysis of HeLa using 10988-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10988-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10988-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10988-1-AP

  IP result of anti-BID (IP:10988-1-AP, 4ug; Detection:10988-1-AP 1:500) with HeLa cells lysate 1280 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, mit Tunicamycin behandelte HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 55 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10988-1-AP bindet in WB, IHC, IF, IP, ELISA BID und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BID fusion protein Ag1445
• Vollständiger Name: BH3 interacting domain death agonist
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC009197
• Gene symbol: BID
• Gene ID (NCBI): 637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

Publikationen

Species	Application	Title
rat	WB	Bioact Mater; A ROS-responsive, self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction.; Authors - Mengyun Yao
mouse	WB	Environ Pollut; Silica nanoparticles inducing the apoptosis via microRNA-450b-3p targeting MTCH2 in mice and spermatocyte cell.; Authors - Guiqing Zhou
rat	WB	Cell Mol Life Sci; Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.; Authors - Katia Fettucciari
human	WB	Int J Cancer; Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.; Authors - Liu Chaoyang C
human	WB	Free Radic Biol Med; Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.; Authors - Gong Ke K
rat	WB	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao

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