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Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10993-1-AP

Synonyms

CYC, CYCS, cyto c, Cytochrome c, cytochrome c, somatic, HCS, THC4



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, Jurkat cells, mouse kidney tissue, rat kidney tissue, C2C12 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10993-1-AP targets Cytochrome c in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, goat, hamster, Hippospongia, pig, rice
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytochrome c fusion protein Ag1455
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene symbol CYCS
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 10993-1-APDownload protocol
IHC protocol for Cytochrome c antibody 10993-1-APDownload protocol
IF protocol for Cytochrome c antibody 10993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

Authors - Tara E Tracy
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
mouseWB

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
humanWB

Autophagy

PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.

Authors - Bo Wang
humanWB

Autophagy

HMGB1 downregulation in retinal pigment epithelial cells protects against diabetic retinopathy through the autophagy-lysosome pathway.

Authors - Lujia Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kazu (Verified Customer) (12-14-2022)

Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse optic nerve
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSC34
FH

Ying (Verified Customer) (04-22-2021)

good antibody. work well with mouse cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
Cytochrome c Antibody Western Blot validation (1:4000 dilution) in (Cat no:10993-1-AP)
FH

Chi (Verified Customer) (09-26-2019)

The antibody works very well with good signal and low background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse embryonic fibroblasts
Cytochrome c Antibody Western Blot, validation (1:2000 dilution) in mouse embryonic fibroblasts (Cat no:10993-1-AP)
FH

Xiaoping (Verified Customer) (10-22-2018)

The signal is good and the size is between 10-15kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse fibroblast
Cytochrome c Antibody Western Blot, validation (1:1000 dilution) in mouse fibroblast (Cat no:10993-1-AP)