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Cytochrome c Polyclonal antibody
Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
524
Cat no : 10993-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, HEK-293 cells, HepG2 cells, C2C12 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue |
Positive IHC detected in | human liver tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse eye tissue |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 491 publications below |
IHC | See 28 publications below |
IF | See 25 publications below |
FC | See 1 publications below |
Product Information
10993-1-AP targets Cytochrome c in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, hamster, sheep, goat, hippospongia |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytochrome c fusion protein Ag1455 |
Full Name | cytochrome c, somatic |
Calculated Molecular Weight | 12 kDa |
Observed Molecular Weight | 12-15 kDa |
GenBank Accession Number | BC009578 |
Gene Symbol | Cytochrome c |
Gene ID (NCBI) | 54205 |
RRID | AB_2090467 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
Protocols
Product Specific Protocols | |
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WB protocol for Cytochrome c antibody 10993-1-AP | Download protocol |
IHC protocol for Cytochrome c antibody 10993-1-AP | Download protocol |
IF protocol for Cytochrome c antibody 10993-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis | ||
Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. | ||
EMBO J Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. | ||
ACS Appl Mater Interfaces Mitochondrial Voltage-Dependent Anion Channel 1-Hexokinase-II Complex-Targeted Strategy for Melanoma Inhibition Using Designed Multiblock Peptide Amphiphiles. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kazu (Verified Customer) (12-14-2022) | Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed.
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FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.
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FH Ying (Verified Customer) (04-22-2021) | good antibody. work well with mouse cells
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FH Chi (Verified Customer) (09-26-2019) | The antibody works very well with good signal and low background.
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FH Xiaoping (Verified Customer) (10-22-2018) | The signal is good and the size is between 10-15kDa.
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