BAD Polyklonaler Antikörper

BAD Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

94

Rezensionen (1)

Kat-Nr. : 10435-1-AP

Synonyme

Bcl2-L-8, BCL2L8, Bcl-2-binding component 6, Bcl2-associated agonist of cell death, BBC6

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 10435-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lymphoma using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lymphoma using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 10435-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BAD (10435-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Lymphomgewebe, humanes Prostatakarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 92 publications below
• IHC: See 4 publications below

Produktinformation

10435-1-AP bindet in WB, IHC, FC (Intra), ELISA BAD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAD fusion protein Ag0688
• Vollständiger Name: BCL2-associated agonist of cell death
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC001901
• Gene symbol: BAD
• Gene ID (NCBI): 572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Publikationen

Species	Application	Title
human	WB	Carbohydr Polym; A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer; Authors - Shaofeng Duan
human	WB	Oxid Med Cell Longev; Cystathionine β-Synthase Regulates the Proliferation, Migration, and Invasion of Thyroid Carcinoma Cells.; Authors - Qi-Ying Jiang
human	WB	Oxid Med Cell Longev; Dihydroartemisinin Modulates Apoptosis and Autophagy in Multiple Myeloma through the P38/MAPK and Wnt/β-Catenin Signaling Pathways.; Authors - Xiuhua Wu
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan
human	WB	Cell Death Dis; CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells.; Authors - Xiaona Mao
human	WB	Oncotarget; Targeting ERK enhances the cytotoxic effect of the novel PI3K and mTOR dual inhibitor VS-5584 in preclinical models of pancreatic cancer.; Authors - Changwen Ning

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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