ASAH1 Polyklonaler Antikörper

ASAH1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 11274-1-AP

Synonyme

AC, Acid ceramidase, Acid ceramidase subunit alpha, Acid ceramidase subunit beta, ASAH

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  WB analysis using 11274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 11274-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASAH1 antibody (11274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, SH-SY5Y-Zellen, SW480-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

11274-1-AP bindet in WB, IHC, IF/ICC, ELISA ASAH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASAH1 fusion protein Ag1799
• Vollständiger Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC016828
• Gene symbol: ASAH1
• Gene ID (NCBI): 427
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.; Authors - Judith Lang
mouse,human	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
human	WB	Hum Mol Genet; Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.; Authors - Myung Jong Kim
mouse	IF	BMC Genomics; Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment; Authors - Yuanmin Jia
mouse	WB	Int J Mol Sci; Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.; Authors - Yu-Ting Su
human	WB	J Dermatol Sci; Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.; Authors - Sawada Eri E