UGCG Polyclonal antibody

• Featured Product
• KD/KO Validated

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 12869-1-AP

Synonyms

Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80

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Validation Data Gallery

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  WB analysis of A375 using 12869-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12869-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 12869-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12869-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12869-1-AP

  IP result of anti-UGCG (IP:12869-1-AP, 4ug; Detection:12869-1-AP 1:500) with A375 cells lysate 3200ug.

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  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12869-1-AP (UGCG antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of frozen mouse kidney tissue fixed with 4% PFA for 24 hours, embedded in OCT, permeabilized with 0.5% Triton X-100 PBS and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, C6 cells, mouse brain tissue, U-251 cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, mouse brain tissue
• Positive IF-Fro detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

12869-1-AP targets UGCG in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGCG fusion protein Ag3530
• Full Name: UDP-glucose ceramide glucosyltransferase
• Calculated Molecular Weight: 394 aa, 45 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC038711
• Gene Symbol: UGCG
• Gene ID (NCBI): 7357
• RRID: AB_10915469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids; Authors - Junchen Liu
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang ; KD Validated
mouse	WB,IF	Cell Mol Biol Lett; UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway; Authors - Shengyu Cui
human, mouse	WB,IHC	Hum Mol Genet; Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.; Authors - Alexandre Henriques
human	WB,IHC	Aging (Albany NY); Identification of long-term survival-associated gene in breast cancer.; Authors - Shipeng Ning
human	IF	iScience; Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.; Authors - Zhiqiang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Will (Verified Customer) (01-31-2019)

good antibody, would order again

• Applications: Western Blot,