Validation Data Gallery
|Positive WB detected in||A375 cells, C6 cells, mouse brain tissue, U-251 cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12869-1-AP targets UGCG in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UGCG fusion protein Ag3530|
|Full Name||UDP-glucose ceramide glucosyltransferase|
|Calculated molecular weight||394 aa, 45 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC038711|
|Gene ID (NCBI)||7357|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.
Aging (Albany NY)
Identification of long-term survival-associated gene in breast cancer.
A Novel Drug Resistance Mechanism: Genetic Loss of Xeroderma Pigmentosum Complementation Group C (XPC) Enhances Glycolysis-Mediated Drug Resistance in DLD-1 Colon Cancer Cells.
Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.
Hum Mol Genet
Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.
Aneuploid cell survival relies upon sphingolipid homeostasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Will (Verified Customer) (01-31-2019)
good antibody, would order again