Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human placenta tissue, mouse colon tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14716-1-AP targets KLF6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF6 fusion protein Ag6440|
|Full Name||Kruppel-like factor 6|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa；42-45 kDa|
|GenBank accession number||BC000311|
|Gene ID (NCBI)||1316|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection
Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes.
Cell Death Dis
Artesunate induces mitochondria-mediated apoptosis of human retinoblastoma cells by upregulating Kruppel-like factor 6.
Cell Death Dis
Hotspot mutant p53-R273H inhibits KLF6 expression to promote cell migration and tumor metastasis.
Cell Death Dis
Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway.
MicroRNA-124 modulates neuroinflammation in acute methanol poisoning rats via targeting Krüppel-like factor-6.
MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Paulo (Verified Customer) (03-01-2019)
Used to perform ChIP-seq in ccRCC cell lines. 20ug of antibody used in 100million cells. No peaks were detected in known target genes and obtained peaks failed to present the consensus KLF6 binding site after motif analysis.