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CREB1 Polyclonal antibody
CREB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12208-1-AP
Validation Data Gallery
|Positive WB detected in||multi-cells/tissue, mouse lung tissue, HEK-293 cells, rat brain tissue, Jurkat cells, COS-7 cells, mouse brain tissue, HeLa cells, PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, HepG2 cells, PC-12 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human cervical cancer tissue, mouse brain tissue, human prostate cancer tissue, human thyroid tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12208-1-AP targets CREB1 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat, chicken, sea cucumbers|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CREB1 fusion protein Ag2852|
|Full Name||cAMP responsive element binding protein 1|
|Calculated molecular weight||341 aa, 35 kDa|
|Observed molecular weight||43-46 kDa|
|GenBank accession number||BC010636|
|Gene ID (NCBI)||1385|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
|Product Specific Protocols|
|WB protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IHC protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IF protocol for CREB1 antibody 12208-1-AP||Download protocol|
|IP protocol for CREB1 antibody 12208-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Aging (Albany NY)
A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.
MicroRNA-23a-3p Inhibits Mucosal Melanoma Growth and Progression through Targeting Adenylate Cyclase 1 and Attenuating cAMP and MAPK Pathways.
Aging (Albany NY)
PTEN participates in airway remodeling of asthma by regulating CD38/Ca2+/CREB signaling.
Cell Death Discov
Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.
Biomed Res Int
LncRNA H19 Upregulation Participates in the Response of Glioma Cells to Radiation.
Cell Death Discov
Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shogo (Verified Customer) (02-22-2019)
Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.