CREB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

142

Reviews (2)

Cat no : 12208-1-AP

Synonyms

Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

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Validation Data Gallery

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  WB analysis using 12208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12208-1-AP

  WB result of CREB1 antibody (12208-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells.

• 

  WB analysis using 12208-1-AP

  WB result of 12208-1-AP (CREB1 antibody) with various lysates at dilution of 1:1,500.

• 

  WB analysis of rat brain using 12208-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12208-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12208-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 12208-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12208-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12208-1-AP

  IP result of anti-CREB1 (IP:12208-1-AP, 4ug; Detection:12208-1-AP 1:2000) with HEK-293 cells lysate 1360 ug.

• 

  IHC staining of mouse brain using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 12208-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CREB1 (12208-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue, multi-cells/tissue, Jurkat cells, COS-7 cells, mouse brain tissue, mouse lung tissue, HeLa cells, PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, HepG2 cells, PC-12 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 133 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 7 publications below
• RIP: See 1 publications below

Product Information

12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, chicken, sea cucumbers
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CREB1 fusion protein Ag2852
• Full Name: cAMP responsive element binding protein 1
• Calculated Molecular Weight: 341 aa, 35 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC010636
• Gene Symbol: CREB1
• Gene ID (NCBI): 1385
• RRID: AB_2245417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

Publications

Species	Application	Title
human,mouse	WB	Cell Metab; Lactate modulates iron metabolism by binding soluble adenylyl cyclase; Authors - Wei Liu
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	Mol Cell; LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation; Authors - Shelby E Compton
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
mouse	WB	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Georgiana (Verified Customer) (01-10-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain tissue, cortex, 20um section

CREB1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, cortex, 20um section (Cat no:12208-1-AP)

FH

Shogo (Verified Customer) (02-22-2019)

Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.

• Applications: ChIP,
• Cell Tissue Type: Brain

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