Galc Polyclonal antibody

• Featured Product
• KD/KO Validated

Galc Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 11991-1-AP

Synonyms

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

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Validation Data Gallery

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  WB analysis using 11991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11991-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11991-1-AP

  IP Result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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  IHC staining of human gliomas using 11991-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: A375 cells, A549 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

11991-1-AP targets Galc in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galc fusion protein Ag3914
• Full Name: galactosylceramidase
• Calculated molecular weight: 77 kDa
• Observed molecular weight: 80 kDa, 30 kDa, 50 kDa
• GenBank accession number: BC086671
• Gene symbol: Galc
• Gene ID (NCBI): 14420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).

Publications

Species	Application	Title
zebrafish	WB,IF	Biochim Biophys Acta; Molecular cloning and knockdown of galactocerebrosidase in zebrafish: New insights into the pathogenesis of Krabbe's disease.; Authors - Daniela Zizioli ; KO Validated
mouse	WB	Glycoconj J; Impact of chronic kidney dysfunction on serum Sulfatides and its metabolic pathway in mice.; Authors - Yosuke Yamada
human	WB,IF	Cell Mol Biol (Noisy-le-grand); Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro.; Authors - Qingxia Tao
		Oncol Rep; GALC gene is downregulated by promoter hypermethylation in Epstein-Barr virus-associated nasopharyngeal carcinoma.; Authors - Yinghai Zhao
mouse	WB	J Mol Neurosci; Synergistic and Superimposed Effect of Bone Marrow-Derived Mesenchymal Stem Cells Combined with Fasudil in Experimental Autoimmune Encephalomyelitis.; Authors - Jing-Wen Yu
mouse	IF	Mol Genet Metab; Intravenous injection of AAVrh10-GALC after the neonatal period in twitcher mice results in significant expression in the central and peripheral nervous systems and improvement of clinical features.; Authors - Mohammad A Rafi