APAF1 Polyklonaler Antikörper

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• KD/KO Validated

APAF1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Kat-Nr. : 21710-1-AP

Synonyme

APAF 1, APAF1, CED4, KIAA0413

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21710-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21710-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21710-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21710-1-AP

  IP Result of anti-APAF1 (IP:21710-1-AP, 4ug; Detection:21710-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 21710-1-AP

  Immunofluorescent analysis of A549 cells using 21710-1-AP (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, COLO 320-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

21710-1-AP bindet in WB, IP, IHC, IF, ELISA APAF1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APAF1 fusion protein Ag16336
• Vollständiger Name: apoptotic peptidase activating factor 1
• Berechnetes Molekulargewicht: 1248 aa, 142 kDa
• Beobachtetes Molekulargewicht: 130-142 kDa
• GenBank-Zugangsnummer: BC136532
• Gene symbol: APAF1
• Gene ID (NCBI): 317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Publikationen

Species	Application	Title
mouse	WB	Theranostics; Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.; Authors - Jinghua Du
human	WB	Oxid Med Cell Longev; Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.; Authors - Ying Wang
human	WB,IHC,IF	Mol Ther Nucleic Acids; LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.; Authors - Huizhe Wu
rat	WB	Cell Biol Toxicol; Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.; Authors - Yujie Zhong
human	WB	Front Cell Dev Biol; Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.; Authors - Bin Chen
human	WB	Cells; FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells; Authors - Yuqi Zhang

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