KU70,XRCC6 Monoclonal antibody

KU70,XRCC6 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

3C12D9

Cat no : 66607-1-Ig

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6



Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human breast cancer tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66607-1-Ig targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KU70,XRCC6 fusion protein Ag21301
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC008343
Gene Symbol XRCC6
Gene ID (NCBI) 2547
RRIDAB_2881967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Protocols

Product Specific Protocols
WB protocol for KU70,XRCC6 antibody 66607-1-IgDownload protocol
IHC protocol for KU70,XRCC6 antibody 66607-1-IgDownload protocol
IF protocol for KU70,XRCC6 antibody 66607-1-IgDownload protocol
FC protocol for KU70,XRCC6 antibody 66607-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF,IP

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
humanWB

Nucleic Acids Res

Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2

Authors - Philip Saumer
humanWB,CoIP

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou
mouseWB

J Nanobiotechnology

Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles.

Authors - Ruijie Qian
humanIF

iScience

WASH interacts with Ku to regulate DNA double-stranded break repair.

Authors - Tao Wang
humanIF

Cell Rep

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells

Authors - Qidong Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Katherine (Verified Customer) (09-25-2023)

Band was clear!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: 293T