CoraLite®594-conjugated KU70,XRCC6 Monoclonal antibody

KU70,XRCC6 Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C12D9

Cat no : CL594-66607

Synonyms

KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66607 targets KU70,XRCC6 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KU70,XRCC6 fusion protein Ag21301
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC008343
Gene Symbol KU70
Gene ID (NCBI) 2547
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Protocols

Product Specific Protocols
WB protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
IF protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
Standard Protocols
Click here to view our Standard Protocols