• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

AKT Polyclonal antibody

AKT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10176-2-AP

Synonyms

AKT pan, AKT 1, AKT (pan), AKT1/2/3, AKT1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of MCF-7 using 10176-2-AP

    WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of HeLa cells using 10176-2-AP

    siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of A549 using 10176-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AKT Polyclonal antibody (10176-2-AP)

    IP experiment of HeLa using 10176-2-AP

    IP result of anti-AKT (IP:10176-2-AP, 4ug; Detection:10176-2-AP 1:15000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human ovary tumor using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human ovary tumor using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human breast cancer using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human breast cancer using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AKT Polyclonal antibody (10176-2-AP)

    IF Staining of mouse brain using 10176-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AKT antibody (10176-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10176-2-AP targets AKT in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish, hamster, sheep, goat, fish, zebra finches
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT fusion protein Ag0213
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_2224574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 10176-2-APDownload protocol
IHC protocol for AKT antibody 10176-2-APDownload protocol
IF protocol for AKT antibody 10176-2-APDownload protocol
IP protocol for AKT antibody 10176-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
View Article
mouseWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
View Article
humanWB

Nat Commun

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

Authors - Pingting Ying
View Article
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
View Article
human,mouseWB

Acta Pharm Sin B

Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2B

Authors - Haowei Liu
View Article
mouseWB

Acta Pharm Sin B

Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment

Authors - Lin Hou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hyopil (Verified Customer) (08-23-2021)

Worked well with western blotting

  • Applications: Western Blot
  • Cell Tissue Type: HAP1
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NSC34