PDK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 18262-1-AP

Synonyms

PDHK1, PDH kinase 1, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

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Validation Data Gallery

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  WB analysis using 18262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 18262-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 18262-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDK1 antibody (18262-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human kidney tissue, human breast cancer tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

18262-1-AP targets PDK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDK1 fusion protein Ag13121
• Full Name: pyruvate dehydrogenase kinase, isozyme 1
• Calculated Molecular Weight: 436 aa, 49 kDa
• Observed Molecular Weight: 46-55 kDa
• GenBank Accession Number: BC039158
• Gene Symbol: PDK1
• Gene ID (NCBI): 5163
• RRID: AB_10598310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.

Publications

Species	Application	Title
human,mouse	WB,IHC	J Clin Invest; PRMT5 mediated FUBP1 methylation accelerates prostate cancer progression; Authors - Weiwei Yan
mouse	WB,IF	Cardiovasc Res; Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism; Authors - Yang Lu
mouse	WB,IF	Cell Death Dis; Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes; Authors - Yuan Yao
mouse	WB	Bioact Mater; Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes.; Authors - Yazhen Li
human	WB,IHC	Cancer Lett; PINK1 deficiency in gastric cancer compromises mitophagy, promotes the Warburg effect, and facilitates M2 polarization of macrophages.; Authors - Ying Xu
human	WB	Redox Biol; Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.; Authors - Tao Zeng