CoraLite®594-conjugated smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-14395

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL594-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200, CoraLite® Plus 488 ZG16 antibody (CL488-67389, Clone: 1A7B9, green).

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Tested Applications

• Positive IF detected in: mouse heart tissue, mouse small intestine tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-14395 targets smooth muscle actin in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: smooth muscle actin fusion protein Ag5886
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC017554
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• RRID: AB_2919805
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publications

Species	Application	Title
	IF	Front Pharmacol; Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment; Authors - Xin-Miao Xue