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  • KD/KO Validated

smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (7)


WB, IP, IF, FC, IHC, CoIP, ELISA, competitive binding assay



Cat no : 14395-1-AP


a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, α SMA, α-sma

Tested Applications

Positive WB detected inmouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, H9C2 cells, mouse heart tissue, mouse small intestine tissue
Positive FC detected inC2C12 cell

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:800-1:3200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14395-1-AP targets smooth muscle actin in WB, IP, IF, FC, IHC, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, monkey, rabbit, zebrafish, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag5886
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC017554
Gene Symbol ACTA2
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.


Product Specific Protocols
WB protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IHC protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IF protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IP protocol for smooth muscle actin antibody 14395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


James (Verified Customer) (09-22-2022)

Used by IF. Very well antibody.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: kidney
smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)

Wu (Verified Customer) (09-08-2022)

Used by IF. Really good signal

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: fibroblast cell
smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish ocular muscles at 3dpf