smooth muscle actin Polyclonal antibody

• Featured Product
• KD/KO Validated

smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay

Conjugate

Unconjugated

Publications

1173

Reviews (4)

Cat no : 14395-1-AP

Synonyms

ACTA2, Alpha SMA, a-SMA, Alpha actin 2, Actin, aortic smooth muscle, intermediate form

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Validation Data Gallery

• 

  WB analysis using 14395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14395-1-AP

  WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.

• 

  WB analysis using 14395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14395-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14395-1-AP

  IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

• 

  IHC staining of human heart using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of stromal tumor using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).

• 

  IHC staining of stromal tumor using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hysteromyoma using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 14395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 14395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 14395-1-AP

  1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue, mouse small intestine tissue
• Positive IF/ICC detected in: C2C12 cells, H9C2 cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 785 publications below
• IHC: See 340 publications below
• IF: See 460 publications below
• FC: See 3 publications below
• CoIP: See 1 publications below

Product Information

14395-1-AP targets smooth muscle actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, monkey, zebrafish, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: smooth muscle actin fusion protein Ag5886
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC017554
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_2223009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publications

Species	Application	Title
rat	IF	Adv Mater; Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring; Authors - Huihui He
mouse,pig	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
mouse	IF	Mil Med Res; Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease; Authors - Guang-Hui Deng
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
rabbit	IF	Bioact Mater; Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing; Authors - Bingcheng Yi
	IHC	ACS Nano; Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair; Authors - Shuang Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (09-22-2022)

Used by IF. Very well antibody.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: kidney

smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)

FH

Wu (Verified Customer) (09-08-2022)

Used by IF. Really good signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: fibroblast cell

smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)

FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish ocular muscles at 3dpf

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