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CoraLite® Plus 488-conjugated pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26411

Synonyms

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan-keratin, Type II keratin Kb5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of HeLa using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human lung cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human lung cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    IF Staining of human lung cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated pan-keratin Polyclon (CL488-26411)

    FC experiment of A431 using CL488-26411

    1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human pan-keratin (CL488-26411) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells, human colon cancer tissue, human lung cancer tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26411 targets pan-keratin in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen pan-keratin fusion protein Ag24184
Full Name keratin 5
Calculated Molecular Weight 590 aa, 62 kDa
GenBank Accession NumberBC024292
Gene Symbol KRT5
Gene ID (NCBI) 3852
RRIDAB_2919208
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 pan-keratin antibody CL488-26411Download protocol
FC protocol for CL Plus 488 pan-keratin antibody CL488-26411Download protocol
Standard Protocols
Click here to view our Standard Protocols