pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 26411-1-AP


pan Cytokeratin, pan-ck, pan-cytokeratin, pan-keratin

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, HeLa cells
Positive IHC detected inhuman oesophagus tissue, human liver tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue, HeLa cells, A431 cells
Positive FC detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:50000-1:200000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26411-1-AP targets pan-keratin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen pan-keratin fusion protein Ag24184
Full Name keratin 5
Calculated Molecular Weight 590 aa, 62 kDa
Observed Molecular Weight 46-58 kDa
GenBank Accession NumberBC024292
Gene Symbol KRT5
Gene ID (NCBI) 3852
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.


Product Specific Protocols
WB protocol for pan-keratin antibody 26411-1-APDownload protocol
IHC protocol for pan-keratin antibody 26411-1-APDownload protocol
IF protocol for pan-keratin antibody 26411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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