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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CD3 Monoclonal antibody

CD3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F3A1

Cat no : CL647-60181

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human colon cancer using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:200, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, red), CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human tonsillitis using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human liver cancer using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®488 CD163 antibody (CL488-16646, green), CoraLite®555 Beta Catenin antibody (CL555-51067, orange).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human tonsillitis using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of Jurkat using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human colon cancer using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 647-conjugated CD3 Monoclonal antib (CL647-60181)

    IF Staining of human colon cancer using CL647-60181

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

Tested Applications

Positive IF-P detected inhuman colon cancer tissue, human tonsillitis tissue, human liver cancer tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60181 targets CD3 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
RRIDAB_2920243
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD3 antibody CL647-60181Download protocol
Standard Protocols
Click here to view our Standard Protocols