CoraLite® Plus 488-conjugated p63 Polyclonal antibody

• Featured Product
• KD/KO Validated

p63 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12143

Synonyms

AIS, B(p51A), B(p51B), CUSP, deltaNp63, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

---

Validation Data Gallery

• 

  IF Staining of A431 using CL488-12143

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 p63 antibody (CL488-12143) at dilution of 1:1000, CL594-Phalloidin (red).

• 

  IF Staining of A431 using CL488-12143

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CL488-12143 (p63 antibody) at dilution of 1:100.

• 

  FC experiment of A431 using CL488-12143

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p63 (CL488-12143) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12143 targets p63 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated Molecular Weight: 680 aa, 77 kDa
• GenBank Accession Number: BC039815
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• RRID: AB_2883081
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011