p63 Polyclonal antibody

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  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12143-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12143-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (TP63 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of Apoptosised HeLa using 12143-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 12143-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12143-1-AP

  IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

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  IP experiment of A431 using 12143-1-AP

  IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell carcinoma using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of A431 using 12143-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

p63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 12143-1-AP

Publications(43)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

TP63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

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Tested Applications

• Positive WB detected in: A431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human tonsillitis tissue, human bowen disease, human lung squamous cell carcinoma tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 12 publications below
• IF: See 16 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12143-1-AP targets p63 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated Molecular Weight: 680 aa, 77 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC039815
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• RRID: AB_10597397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H3D4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB,IP,ChIP	Nucleic Acids Res; Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.; Authors - Yongsheng Huang ; KD Validated
human	IF	Cell Rep; A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma; Authors - Yue Li
mouse	IHC	Oncogene; TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.; Authors - Samera H Hamad
human	IF	Elife; Chloride channels regulate differentiation and barrier functions of the mammalian airway.; Authors - Mu He
human	IF	Stem Cell Res Ther; Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2; Authors - Zhilin Sui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Kui (Verified Customer) (09-19-2019)

Best P63 Ab I can find so far.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Esophagus

p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)