p63 Polyclonal antibody

• Featured Product
• KD/KO Validated

p63 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (2)

Cat no : 12143-1-AP

Synonyms

AIS, B(p51A), B(p51B), CUSP, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

---

Validation Data Gallery

• 

  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12143-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 12143-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 12143-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12143-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (TP63 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of A431 using 12143-1-AP

  IP Result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.

• 

  IP experiment of A431 using 12143-1-AP

  IP Result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.

• 

  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, mouse heart tissue, human heart tissue, U-937 cells, Apoptosised HeLa cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human tonsillitis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, human lung cancer tissue

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12143-1-AP targets p63 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated molecular weight: 680 aa, 77 kDa
• Observed molecular weight: 63-68 kDa
• GenBank accession number: BC039815
• Gene symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Publications

Species	Application	Title
human	WB	Cancer Res; BCL-2 is a Therapeutic Target For Hypodiploid B-Lineage Acute Lymphoblastic Leukemia.; Authors - Ernesto Diaz-Flores
human	IF	Exp Eye Res; Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling.; Authors - Lihua Hu
human	IF	Cell Rep; ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia.; Authors - Maximilian Haas
human	WB	J Exp Clin Cancer Res; ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.; Authors - Mengzhao Zhang
human	IF	STAR Protoc; Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells; Authors - Vincent Manna
human	IF	BBA Adv; The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia.; Authors - Vincent J Manna

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Kui (Verified Customer) (09-19-2019)

Best P63 Ab I can find so far.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Esophagus

p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)

Related Products

P53 Polyclonal antibody

10442-1-AP

GLUT1 Polyclonal antibody

21829-1-AP

XIAP Polyclonal antibody

10037-1-Ig

DLX3 Polyclonal antibody

13261-3-AP

YAP1 Polyclonal antibody

13584-1-AP

VDR Polyclonal antibody

14526-1-AP