ChromoTek Nano-Secondary® anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 [CTK0105, CTK0106]

Validation Data Gallery

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  Immunofluorescence analysis of HeLa cells stained with mouse IgG2b anti-Tubulin beta antibody and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, orange). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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  HEK-293 cell lysates were subjected to SDS-PAGE followed by multiplex western blot analysis with 3 mouse primary antibodies including anti-ATP50 (66696-1-Ig), anti-AIF (67791-1-Ig), and anti-actin (66009-1-Ig). Primary antibodies were detected using 3 mouse IgG subclass-specific nano-secondary reagents including Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 750 (smsG1CL750-1, white), Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 488 (smsG2aCL488-1, green), and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, red).

Nano-Secondary® anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

Cat No. smsG2bCL555-1

Publications(1)

Applications

IF, WB

Reactivity

mouse IgG2b

Host

Alpaca

Conjugate

CoraLite® Plus 555

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

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Product Information

Nano-Secondary® anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
• Applications: IF, WB
• Host: Alpaca-derived, recombinantly produced in bacteria
• Species Reactivity: Mouse IgG2b Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3, IgM
• Target/Specificity: Fc-fragment of mouse IgG2b
• Physical State: Liquid
• Conjugate: CoraLite® Plus 555
• Suggested Dilution: Immunofluorescence 1: 500 - 1:1,000; Western blot 1: 500 - 1:1,000
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Secondary Nanobody
• Class: Recombinant
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG1, IgG2a, IgG2c, IgG3, and IgM
• Immunogen: Purified mouse IgG2b
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Clones: CTK0105 (VHH0275), CTK106 (VHH0288)
• Affinity (KD): CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
• RRID: AB_2941315
• Excitation / Emission: Excitation max: 554 nm, Emission max: 570 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Medicina (Kaunas); Immunological Landscape of Non-Melanoma Skin Neoplasms: Role of CTLA4+IFN-γ+ Lymphocytes in Tumor Microenvironment Suppression; Authors - Silvana Karabatić Knezović