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ChromoTek Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117]

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

Applications

IF, FC

Conjugate

CoraLite® Plus 555

Host

Alpaca

Reactivity

human IgG

Cat no : shuGCL555-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation Data Gallery

  • Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.

    Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.

  • Immunofluorescence analysis of transgenic CHO-PD1 cells. PFA-fixed cells were stained with anti-human PD1 (Pembrolizumab biosimilar), Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, red), and DAPI (grey).

    Immunofluorescence analysis of transgenic CHO-PD1 cells. PFA-fixed cells were stained with anti-human PD1 (Pembrolizumab biosimilar), Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, red), and DAPI (grey).

  • Schematic representation of anti-human IgG Nano-Secondary binding to its target. Human IgG primary antibody (blue) is bound with two identical monoclonal human IgG Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the human IgG primary antibody.

    Schematic representation of anti-human IgG Nano-Secondary binding to its target. Human IgG primary antibody (blue) is bound with two identical monoclonal human IgG Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the human IgG primary antibody.

Product Information

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

ApplicationsIF, FC
DescriptionNano-Secondary® alpaca anti-human IgG, Fc-specific recombinant VHH reagent uses a novel class of human IgG-specific antibodies. This secondary antibody product consists of a Nanobody that binds to human IgGs (IgG1, IgG2, IgG3, IgG4) with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species apart from slight reactivity to macaque serum, details see below.
HostAlpaca
Species ReactivityReactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum.
No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep.
Physical StateLiquid
ConjugateCoraLite® Plus 555
Suggested DilutionImmunofluorescence 1:500 - 1:1000
Flow cytometry: 1µL per 1x106 cells
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD)< 10 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage Condition Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
RRIDAB_3075303
NoteThis product is for research use only, not for diagnostic or therapeutic use