ChromoTek Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 [CTK0117]

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

Applications

IF, FC

Reactivity

human IgG

Host

Alpaca

Conjugate

CoraLite® Plus 647

Publications

1

Cat no : shuGCL647-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

---

Validation Data Gallery

• 

  Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 [CTK0117] (magenta, shuGCL647-2 ). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.

• 

  Immunofluorescence analysis of transgenic CHO-TIM3 cells. PFA-fixed cells were stained with anti-human TIM3 (Cobolimab biosimilar), Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 [CTK0117] (gray) and DAPI (blue).

• 

  Flow cytometry analysis of 1X10^6 human peripheral blood mononuclear cells (PBMCs) stained with anti-human CD20 ( Rituximab biosimilar) and Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 [CTK0117) (shuGCL647-2). Cells were co-stained with CoraLite® Plus 488 conjugated anti-human CD19 antibody.

• 

  Dot blot assay demonstrates that anti-human IgG Nano-Secondary (CTK0117) has no cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse, rabbit, rat, and sheep serum. It shows slight cross-reactivity to macaque serum.

• 

  Schematic representation of anti-human IgG Nano-Secondary binding to its target. Human IgG primary antibody (blue) is bound with two identical monoclonal human IgG Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the human IgG primary antibody.

---

Product Information

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-human IgG, Fc-specific recombinant VHH reagent uses a novel class of human IgG-specific antibodies. This secondary antibody product consists of a Nanobody that binds to human IgGs (IgG1, IgG2, IgG3, IgG4) with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species apart from slight reactivity to macaque serum, details see below.
• Applications: IF, FC
• Host: Alpaca
• Species Reactivity: Reactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum.; No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep.
• Target/Specificity: Fc-fragment of human IgG (IgG1, IgG2, IgG3, IgG4)
• Physical State: Liquid
• Conjugate: CoraLite® Plus 647
• Suggested Dilution: Immunofluorescence 1:500 - 1:1000; Flow cytometry: 1µL per 1x10^6 cells
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Secondary Nanobody
• Class: Recombinant
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse, rabbit, ; rat, and sheep serum. ; Shows slight cross-reactivity to macaque serum.
• Immunogen: Purified human IgG
• Clonality: Monoclonal Nanobody
• Clones: CTK0117 (VHH1118)
• RRID: AB_3075304
• Affinity (KD): < 10 pM
• Excitation / Emission: Excitation max: 654 nm, Emission max: 674 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

Publications

Application	Title
	EBioMedicine; Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone; Authors - Kirsty Hodgson