cGAS Recombinant antibody

cGAS Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1

Cat no : 82869-1-RR

Synonyms

C6orf150, cGAS, h-cGAS, MB21D1

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Validation Data Gallery

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  WB analysis using 82869-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, THP-1 cells, MCF-7 cells, NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82869-1-RR targets cGAS in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: cGAS fusion protein Ag23455
• Full Name: chromosome 6 open reading frame 150
• Calculated Molecular Weight: 496 aa, 54 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC113608
• Gene Symbol: C6orf150
• Gene ID (NCBI): 115004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.