cGAS Recombinant antibody, PBS Only

• 

  WB analysis using 82869-1-RR (same clone as 82869-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 82869-1-RR (same clone as 82869-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug cGAS Recombinant antibody (82869-1-RR, Clone:1H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82869-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82869-1-RR against Human cGAS were performed. The affinity constant is 0.124 nM.

cGAS Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82869-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Add to Basket

Please visit your regions distributor:

---

Proteintech Guarantee

---

---

Product Information

82869-1-PBS targets cGAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: cGAS fusion protein Ag23455
• Full Name: chromosome 6 open reading frame 150
• Calculated Molecular Weight: 496 aa, 54 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC113608
• Gene Symbol: cGAS
• Gene ID (NCBI): 115004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N884
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.