c-MYC Monoclonal antibody, PBS Only

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  WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67447-1-Ig (same clone as 67447-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

  1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 67447-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Freight/Packing: -

Quantity

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Product Information

67447-1-PBS targets c-MYC in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-MYC fusion protein Ag17519
• Full Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000141
• Gene Symbol: MYC
• Gene ID (NCBI): 4609
• RRID: AB_2882681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01106
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.