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  • KD/KO Validated

c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

3D9C12

Cat no : 67447-1-Ig

Synonyms

bHLHe39, c Myc, c-MYC, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64



Tested Applications

Positive WB detected inMDA-MB-231 cells, HT-29 cells, 4T1 cells, PC-3 cells, K-562 cells, HL-60 cells, Raji cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67447-1-Ig targets c-MYC in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-MYC fusion protein Ag17519
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2882681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 67447-1-IgDownload protocol
IHC protocol for c-MYC antibody 67447-1-IgDownload protocol
FC protocol for c-MYC antibody 67447-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
humanWB

Drug Resist Updat

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Authors - Shuai Wang
  • KD Validated
human,mouseWB

Nat Commun

ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer

Authors - Shuang Shang
humanWB

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
human,mouseWB

Acta Pharm Sin B

Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors.

Authors - Yang Zhou
humanIP

Cell Death Differ

E3 ligase TRIM25 ubiquitinates RIP3 to inhibit TNF induced cell necrosis.

Authors - Pucheng Mei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanis (Verified Customer) (03-15-2022)

Antibody was strong, clean, and specific in our application.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000